The effect of nitrogen deposition on flowering

write up a initial plan to set up an experiment about the impact of N deposition on the flowering in grassland habitats as the following order below/
1- The detailed methods for this experiment (The rate of nitrogen for each plot will be applied at 20 g m-2 yr-1).
2- For the area of this study, I already have chosen the study area.
3- How many samples need to be taken from each plot?.
4- The main equipments for this experiment, how many pots?.
5- The hypothesis of this experiment (the questions that to be answered for this study).