Techniques that are used to study a known protein

Choose one of the following techniques that are used to study a known protein

1. Protein expression systems
2. Southwestern and Northwestern
3. Fluorescent Confocal Microscopy to study protein co-localization
4. Tandem Affinity Purification
5. Single molecule protein sequencing methodologies
   You will
   a) Give an overview of the technique.
   a. Why is it used
   b. Give a general protocol that highlights the major steps in the general procedure
   b) What advantages does the technique have for providing relevant information about a particular
   protein?
   c) What are the limitations of the technique?
   d) What are the major findings the technique provides when using it to study a particular protein