Chemistry Lab Analysis

  1. How much of a 10x buffer should be added to 900ml of water to make a 1x solution?
  2. How much of a 5x buffer and how much water should be added to make 1 L of a 1x solution?
  3. How much of a 100x buffer and how much water should be combined to make 50 ml of a 1x
    solution?
  4. You want to make a solution that is 1x TE AND 10% glycerol in the same solution. You have
    the following stock solutions:
    10x TE
    50% Glycerol
    Water
    How much of each should be combined to make 100 mls?
  5. After viewing and analyzing your gel, draw a map of your plasmid showing all BamHI and XbaI
    sites and the distances between them.