Cell Biology Act

 


Background

What an incredible medical discovery we have made together! A contagious cancer is one of the rarest

diseases throughout the galaxy. If left untreated, the cancer will not only spread from the lungs to other

organs but will spread from sick gliders to healthy gliders.

Fortunately, we have collected the data to determine how the tumor cells differ from healthy cells. The

elevated concentration of Growth Factor L in the blood of spotted gliders provides a clue to the mutations

responsible for the cancer. We may be able to use this information to devise a treatment that targets

tumor cells.

Use the following questions to guide your work:

● Which receptors in the cell membrane bind Growth Factor L? (Appendix 1)

● Which proteins in the cytoplasm are activated when a receptor binds Growth Factor L? (Appendix

2)

● Should we treat the cancer of spotted gliders with a drug or a virus? (Appendix 3)

Universally, in your debt,

The AI

You have been asked to identify the cellular component contributing to the spotted gliders’ cancer

 

and chose a treatment.

 

Appendix 1

 

Which receptors in the cell membrane bind to

 

Growth Factor L?

 

Cancer—the uncontrolled growth of abnormal cells—results from a malfunction in a signaling pathway.

The figure below shows a signaling pathway that regulates cell division in spotted gliders.

When the signaling pathway is inactive, a protein called CDI2 is active and prevents the cell from dividing.

All other proteins (R1, KR1, KR2, KR3, and CDP1) are inactive form.

When the signaling pathway is in the active state, the following steps occur:

1) A signal (SR1) binds to a receptor (R1), activating this receptor through phosphorylation.

2) The active form of R1 or R2 binds and phosphorylates a kinase (KR1).

3) The active form of KR1 binds and phosphorylates a second kinase (KR2).

4) The active form of KR2 binds and phosphorylates a third kinase (KR3).

5) The active form of KR3 interacts directly with two proteins, CDP1 and CDI2. KR3 activates

CDP1, which enables CDP1 to promote rapid cell division. KR3 inactivates CDI2, preventing

CDI2 from slowing cell division.

 

When examining the spotted gliders, we discovered an elevated concentration of a second type of signal,

which Phygaran scientists called Growth Factor L (abbreviated as GF-L). According to my database,

GF-L only reaches these concentrations during the embryonic stage, early in the development of spotted

gliders, when cells rapidly divide.

By experimenting with some cells from a spotted glider, you discovered that GF-L binds to receptors in

the membrane of a tumor cell. We might presume that these receptors are the same as R1, which would

explain how GF-L promotes cell division (see Figure 1). However, after you left the Intergalactic Wildlife

Sanctuary, I analyzed the membrane of tumor cells and discovered a second type of receptor, R2, that

has a similar structure to R1. According to research conducted on Phygaris, spotted gliders usually

produce R2 as embryos when rapid cell division creates new tissues and organs. R2 is not usually

expressed in adult gliders.

We need to understand GF-L's role in the cancer of spotted gliders. First, we need to determine

whether GF-L binds to R1 or R2. This knowledge will help us identify the cause of the cancer.

We will follow three steps to answer the question, “Is Growth Factor L binding to Receptor 1 or 2?”

Step 1: Anticipate your analysis. Determine what you should observe if GF-L binds to R1, R2, both

receptors, or neither receptor. This step will help us identify the evidence needed to build an argument in

Step 3.

Step 2: Model the effects of GF-L on the activity of R1 and the activity of R2. Determine how the

 

presence of GF-L affects the activity of R1 and the activity of R2. This step gives us the evidence needed

to build an argument in Step 3, when we will conclude whether GF-L binds to R1, R2, both receptors, or

neither receptor.

Step 3: Weigh the evidence and conclude if GF-L binds to R1, R2, both receptors, or neither

receptor. Construct an argument to answer the question “Does GF-L bind only to R1, only to R2, both

receptors, or neither receptor?” Your argument should draw on your answers in Steps 1 and 2.

 

Sample Answer

 

 

 

 

 

 

The Cellular Component Contributing to Spotted Gliders’ Cancer

 

 

1. Which receptors in the cell membrane bind Growth Factor L?

 

To determine which receptors bind to Growth Factor L (GF-L), we must analyze the provided information and anticipate the effects.

Anticipated Analysis (Step 1):

If GF-L binds to R1, the activity of R1 will increase, leading to a cascade of phosphorylation events in the signaling pathway. The activity of R2 will remain unchanged.

If GF-L binds to R2, the activity of R2 will increase, also leading to the activation of the signaling pathway. The activity of R1 will remain unchanged.

If GF-L binds to both R1 and R2, the activity of both receptors will increase.

If GF-L binds to neither receptor, the activity of both R1 and R2 will remain unchanged.

Modeled Effects (Step 2):

  •  

The presence of R2 in adult gliders is an abnormal finding, as it is usually only expressed during the embryonic stage of rapid cell division. The elevated concentration of GF-L—which is also typically found only during the embryonic stage—binds to these aberrantly expressed R2 receptors. This binding activates the signaling pathway, leading to the uncontrolled cell division characteristic of cancer.

The cellular component contributing to the cancer is the abnormal expression of Receptor 2 (R2) in the cell membranes of adult gliders.

 

2. Which proteins in the cytoplasm are activated when a receptor binds Growth Factor L?

 

According to Appendix 2, the signaling pathway is activated through a series of phosphorylation events initiated by a receptor binding to a signal. When GF-L binds to R2, it activates the following proteins in the cytoplasm:

KR1 (Kinase 1): Activated when bound and phosphorylated by R2.

KR2 (Kinase 2): Activated when bound and phosphorylated by active KR1.

KR3 (Kinase 3): Activated when bound and phosphorylated by active KR2.

CDP1 (Cell Division Promoter 1): Activated directly by active KR3, which then promotes rapid cell division.

Additionally, KR3 inactivates the protein CDI2 (Cell Division Inhibitor 2), which normally prevents the cell from dividing. The combined effect of activating CDP1 and inactivating CDI2 results in uncontrolled cell division.

 

Proposed Treatment

 

 

3. Should we treat the cancer of spotted gliders with a drug or a virus?

 

Based on the information provided, the best course of treatment for the spotted gliders' cancer would be a drug.

A drug can be designed as a small molecule inhibitor that specifically targets and blocks the activity of the activated proteins within the cytoplasm. Targeting KR1, KR2, or KR3 would be the most effective strategy. By inhibiting any one of these kinases, we could stop the signaling cascade, thereby preventing the activation of CDP1 and the inactivation of CDI2. This would halt the uncontrolled cell division without affecting other cellular processes that do not rely on this specific pathway.