Biology

Just watch the video or check ( The Meselson-Stahl Experiment) and answer those questions please
1 .In Meselson-stahl’s experiment, what is the purpose using isotope N14 and N15?
2.Assuming E.coli have heavy form of DNA at generation zero. You put the E.coli into N14 medium. After one generation, what is the percentage of heavy form? light form? intermediate form?
3.After 3 generations, what is percentage of heavy form, light form and intermediate form (use semi-conservative model)?