biochem;

biochem; QUOTE DIRECTLY FROM THE PAPER. You need to use your own words. Read the attached article: “ A Mitochondrial Pyruvate Carrier Required for Py ruvate Uptake in Yeast, Drosophila, and Humans,” Science , 337 , p. 96 - 100 and answer the following questions. The authors describe experiments performed to identify the carrier protein involved in pyruvate uptake into the mitochondria. 1) How did the authors demonstrate that the Mpc1 protein was located in the mitochondrial inner membrane? 2) Describe how the authors demonstrate d that Mpc1 protein function was conserved through evolution. 3) The Drosophila that lacked dMPC1 (dMPC1 - ) died q uickly on a sugar only diet (Figure 2A). What does this suggest about sugar metabolism in these flies? How did measurement of metabolites in Figure 2F, 3A and 3B help the authors understand why the dMPC1 - flies were dying? 4) Even without a pyruvate carri er to transport pyruvate from the cytosol to the matrix, the mitochondri a can still have some pyruvate in the matrix. Describe one way that pyruvate can be generated in the mitochondrial matrix in the absence of Mpc1. 5) Describe the experiment in this pa per that most directly demonstrates that Mpc1 is a pyruvate carrier. PLACE THIS ORDER OR A SIMILAR ORDER WITH US TODAY AND GET AN AMAZING DISCOUNT :)