Alkaline Phosphatase Proposal

These experiments should be well thought out so that you might accomplish them in a few weeks time and with a reasonable amount of protein. This means approximately 1 mg/mL and maybe 1-2 mL. You can test any and all of the following samples: wt, C168S, C286S, D51N, K328I and one other point mutation of your choice. You may use any of the techniques discussed in class but remember you should include some comparison between positive and negative controls. For instance, when investigating the stability of the protein to thermal denaturation, you should plan on measuring shifts in Tm between wildtype and your mutant. The proposal should be written like a materials and methods section, but in the future tense (i.e., passive voice). There should be a clear logic between experiments tied to specific goals (or specific aims) you decide upon with your lab partners.
The entire proposal should be no more than 2 pages (excluding references) and should focus on the "why" of your proposed experiments. Why are you doing them? What new data will they bring? What questions will they answer? It's assumed that you know, or can find out about, the "how" of your proposed experiments - like how will you do them in detail.